Result . a method of multiplex pcr based on both chimeric and universal primers was established . a fluorescent quadriplex str system , including d1s1612 , d9s2026 , d20s161 and d6s477 , was developed on the basis of the multiplex pcr 建立了dis1612 、 d952026 、 d205161 、 d65477str基因座熒光標(biāo)一記嵌合引物復(fù)合擴(kuò)增毛細(xì)管電泳激光自動(dòng)分析體系,同時(shí)證明嵌合引物復(fù)合擴(kuò)增方法與毛細(xì)管電泳激光自動(dòng)分析方法相兼容。
The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci , to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method , and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment 目的本課題旨在探索一種新的str基因座復(fù)合擴(kuò)增方法,我們稱(chēng)為嵌合引物str復(fù)合擴(kuò)增法。應(yīng)用熒光標(biāo)記毛細(xì)管電泳激光自動(dòng)檢測(cè)技術(shù)平臺(tái),建立一套新的法醫(yī)str基因座復(fù)合擴(kuò)增體系,并按照美國(guó)dna分析方法技術(shù)工作組( thetechnologyworkinggroupondnaanalysismethods , twgdam )的指導(dǎo)方案進(jìn)行法醫(yī)學(xué)實(shí)用性研究。
The use of chimeric primers provides a method for primer design that eliminates the multiple optimization steps involved in developing multiplex pcr . our fluorescent quadriplex str system can be analyzed by capillary electrophoresis and provide a new sources of typing regent for this technique platform with laser detecting system 結(jié)論建立了法醫(yī)四個(gè)遺傳標(biāo)記dis1612 、 d952026 、 d205161 、 d65477新的熒光標(biāo)記嵌合引物復(fù)合擴(kuò)增體系,證明該體系可成功應(yīng)用于熒光標(biāo)記毛細(xì)管電泳激光自動(dòng)分析檢測(cè)平臺(tái)。