Case of myiasis caused by the larva hypoderma bovis 牛皮蠅幼蟲所致蠅蛆病1例1
And hypoderma de not . synthetical taxology )部分種進行較系統的研究。
The expression products were tested by wester - blotting , it demonstrated the fusion proteins can be recognized by positive serum of hypoderma sp 通過westem ? blotting分析表達產物,表明表達產物具有免疫活性,能被制備的陽性血清所識別。
Total rna was extracted from the second stage larve of hypoderma sp , the 713bp of hypodermin c ( hc ) and 700bp of hypodermin a ( ha ) gene were amplified by rt - pcr 2 從皮蠅二期幼蟲中提取獲得總rna ,利用rt - pcr技術擴增獲得了大小為713bp的hypoderminc和大小為700bp的hypodermina基因。
6 . hc and ha antigens were separated by sds - page from nature hypoderma sp larve , then immunized rabbitsrespectivelly , hc and ha positive serum were preparated . 7 從天然皮蠅幼蟲中提取了皮蠅抗原,通過sds一page分離獲得了hypoderminc和hypodermina天然抗原,用此抗原免疫家兔,制備了皮蠅hypoderminc和hypodermina陽性血清。
The kind of fungi fruit bodies develop on recently dead for intrusion or decayed plant materials . the lophodermium and hypoderma fungi collected from the huangshan mountains were isolated by tissue isolation techniques and culture characters data of 7 species were obtained 以組織分離法對采自安徽皖南山區的斑痣盤菌活菌材料進行分離培養,獲得7種散斑殼菌及皮下盤菌的培養性狀資料。
Total rna was extracted from the second stage larve of hypoderma sp , then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer . the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software 本試驗的目的旨在進行hypodeminc ( hc )和hypodermina ( ha )基因的克隆、測序、構建重組表達載體并誘導表達,獲得重組抗原,以解決天然抗原的不足并為診斷和免疫試劑的產業化奠定基礎。