The expression efficiency difference between ped5 and pcdhfrl , a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production , was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters . when analyzed in transient expression , ped5 showed a little more protein produciton than pcdhfrl . however , in continuous expression , when serum concentration was lessened to slow down cell proliferation , ped5 expressed 3 . 1 times more reporter proteins than pcdhfrl , which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie , making ped5 a good expression vector for foreign protein production 應用人-干擾素( ifn - )和人分泌型堿性磷酸酶( seap )基因作為報告基因,對含有巨細胞病毒即早期啟動子( p _ ( cmv - ie ) )的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較,發現對于瞬時表達, ped5略好于pcdhfr1 ;在穩定表達中,通過降低血清濃度,使細胞增殖緩慢,這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
百科解釋
The Downs process is an electrochemical method for the commercial preparation of metallic sodium, in which molten NaCl is electrolyzed in a special apparatus called the Downs cell.