Computer - assisted analysis revealed there was a signal peptide near the n - terminal of this protein 軟件預測0rf132有一個信號肽序列( signalpeptide ) ,最可能的切割位點在和r20之間。
Era contains 97 possible cutting sites that can be recognized by thermolysin which belong to the same family with yggg Thermolysin (嗜熱菌蛋白酶)對era有97個可能的切割位點;嗜熱菌蛋白酶,和yggg同屬一個蛋白酶家族。
The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod 結果表明同源性分別達到98和97 ,并且在ha1切割位點有多個堿性氨基酸的插入序列,證明其為強毒株。
The cleavage site of putative signal peptide was predicted to occur between 28 ( ala ) and 29 ( ala ) thus the putative mature form of the protein composed of 92 amino acids with a molecular weight of 9 . 2 kda 推測的最可能的信號肽切割位點位于第28 ( alal和29 ( alal個氨基酸之間。椎測的成熟肽共有92個氨基酸,分子量9 zkda 。
Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv , the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr 由于硫氧還蛋白和抗菌肽之間設計了腸激酶( enterokinase )切割位點和cnbr切割位點,通過對該表達的融合蛋白的切割,可得到目標抗菌肽cmiv突變體多肽分子。
2 . the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein , encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids . 19 highly conserved , potential glycosylation sites and 17 cysteines residues were characterized with si protein , homology analysis showe that there were gene deletion - S1基因:其全序列共1614bp (從起始密碼子atg到s前體蛋白裂解位點) ,編碼537個氨基酸,其氨基端有一編碼18個氨基酸的信號肽序列,第12 13位氨基酸殘基構成了信號肽的切割位點, 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。