Experimental study on mice immunized with recombinant vaccinia virus 重組痘苗病毒免疫小鼠的實驗研究
The researchers began by removing two genes from a vaccinia virus that are necessary for its growth in normal cells 研究人員們首先將痘苗病毒中在正常細胞中生長所必須的兩個基因去除。
By using aa 385 - 365 fragment , an elisa system for the evaluation of post - e2 - vaccination humoral immune responses was also established , and was successfully applied to recombinant vaccinia virus - and dna - based vaccine research 利用aa385 - 565片段,建立了e2疫苗免疫后抗體反應的elisa評價體系,并已成功用于重組痘苗病毒疫苗和dna疫苗的研究。
During daily monitoring for the onset of contact vaccinia symptoms , 1 person had a rash and 1 had fever , but neither person had vaccinia virus infection ; all other potential contacts remained healthy with no nosocomial transmission 對開始接觸有牛痘癥狀的日常監護期間,一人出現皮疹一人發熱,但沒有人感染牛痘病毒,所有其他可能性的非醫院的傳染接觸依然健康。
May 18 , 2007 ? in the first documented us case of eczema vaccinatum ( ev ) since 1988 , reported in the may 18 issue of the morbidity and mortality weekly report , secondary transmission of vaccinia virus occurred from a father vaccinated for smallpox to his child 2007年5月18日,發病率和死亡率每周報道5月18日刊報道,美國首例牛痘性濕疹于1988年被報道,繼發的痘苗病毒傳播發生在一位接種了天花疫苗的父親傳染給了兒子。
In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv 本研究采用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,并獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
To answer this question , a bispecific , trifunctional antibody constructs which can not only target block virus incorporated rca , but also can induce complement activation by it ' s fc fragment were designed and constructed and iv the role of this kind of bispecific antibody in virus neutralization was studied . 1 . to test our idea , human immunodeficient virus ( hiv ) and enveloped extracellular virus ( eev ) of vaccinia virus ( vv ) were selected in our study because of their complex immune evasion stratiges , their threaten to humans , and because both these two kinds of virus can escape complement attack by incorporating host rca into their envelope 以嚴重危害人類健康,且具有復雜免疫逃避機制的有包膜的hiv病毒及痘苗病毒的eev病毒為研究對象,首先對它們逃避補體攻擊的現象進行了驗證,探討了宿主膜補體調節蛋白cd55 、 cd59與hiv病毒及eev病毒免疫逃避的關系,評價了病毒結合的這兩種補體調節蛋白作為本研究提出的,通過消除病毒逃避補體攻擊的機制來恢復病毒對補體攻擊的敏感性,提高補體抗病毒效率這一抗病毒策略的靶點的可能性。