As explants , were infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring the expression vectors pbgsb and pbglsb respectively and co - cultured for 3 days in darkness on the culture medium ( ms + 0 . 1 mg / l 6 - ba + o . l mg / l naa + 0 . 7 % agor ph 5 . 7 ) Ling inaa )中暗培養(yǎng)三天后,移入加入抗生素100mg lkan和500mg幾cr的誘芽培養(yǎng)基,于組培室中進(jìn)行誘芽和抗性篩選。
Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba , cppu , tdz or kt ) alone or in combination with naa or iba , and the adventitious buds regeneration rate was evaluated . 以榨菜子葉、真葉為外植體進(jìn)行離體培養(yǎng),用ba 、 cppu 、 tdz和kt等細(xì)胞分裂素和生長素naa 、 iba組合誘導(dǎo)子葉、真葉再生不定芽試驗(yàn)。
Anti - keratin auto antibody ( ak auto ab ) is accepted generally as a significant component of natural auto antibody ( naa ) which is found in human as well as vertebrate , and is of characteristic of polyreactivity , low avidity , extensive connectivity and shorting of specificity to the species 天然自身抗體( naa )廣泛存在于人和脊椎動物體內(nèi),具有多反應(yīng)性、親和力低、締和性廣及種屬特異性差的特點(diǎn)。
This paper deals mainly with the extraction of geological and hydrogeological information from deep strata through the comparative analysis of major elements , minor elements and trace elements in rocks and in relics of dried fountains by means of neutron activation analysis ( naa ) 摘要利用中子活化測試結(jié)果,對巖石及泉水乾涸殘馀物中常量及微量元素的進(jìn)行分析對比,以?解深層巖體有關(guān)的地質(zhì)與水文地質(zhì)信息。
Is one of the most valuable and most widely cultivated small fruits in the world . while the global soil salt stress and drought caused more and more serious affection on strawberry production . it is necessary to culture salt and drought resistant strawberry cultivars via foreign gene transformation 以不同品種草莓(達(dá)賽和豐香)花藥為外植體,在附加6 - ba與2 , 4 - d 、 6 - ba與naa的ms培養(yǎng)基上進(jìn)行培養(yǎng),均可誘導(dǎo)出愈傷組織。
Calli were induced by culturing hypocotyls of pepper in the ms medium supplemented with 2 . 0mg / l 6 - ba and l . omg / l naa . the diploid wound callus was treated by adding colchicines into the medium and by immersing into colchicines solution for different concentrations and periods 通過將不同濃度的秋水仙堿加入培養(yǎng)基和不同濃度的秋水仙堿溶液浸泡兩種方法處理愈傷組織,從而篩選出最佳的誘導(dǎo)方法和最佳的秋水仙堿濃度和處理時(shí)間。
( 3 ) regenerating plantlets were obtained via somatic embryo , which derived from hairy root . the effects on the induction of somatic embryos from hairy root were investigated using orthogonal test . the best somatic embryo induction was obtained on ms + 0 . 5mg / l naa + 0 . 5mg / l kt + 30g / l sucrose 單獨(dú)使用naa很難誘導(dǎo)出體胚,單獨(dú)使用kt或kt與naa配合使用均能誘導(dǎo)出體胚, naa 、 kt和蔗糖對體胚誘導(dǎo)的影響作用依次是t a蔗糖。
But cluster buds can not be obtained from the callus of stems and embryo . it has likely relate to the inner structure of callus . lt is very difficult to get embryoid from mature embryo and its inducing rate is very low , and only 11 . 5 % in the media containing n6 + ba 1 . 5mg / l + naa 0 . 5mg / l + sucrose 30g / l + agar 8g / l + ac 2g / l which is the best media for gingo tissue culture 而莖段和胚誘導(dǎo)的愈傷組織基本上沒有叢生芽發(fā)生,這可能和不同愈傷組織的內(nèi)部結(jié)構(gòu)有關(guān)。成熟胚的胚狀體誘導(dǎo)率較低,誘導(dǎo)相當(dāng)困難,在n6 bai
Bap performed more important function than kt in differentiation of tall fescue embryogenic calli , but better results could be achieved with combination of 2mg / l bap and 0 . 5mg / l kt . at this cytokinin level , 0 . 5mg / l naa was recommended to obtain the highest callus regeneration frequency . plant regeneration could be evidently boosted when embryogenic calli were pre - differentiated on high - osmoticum medium with 60g / l sucrose , and / or when the pre - differentiated compact calli were differentiated on differentiation medium solidified with l0g / l agar 高羊茅胚性愈傷組織分化時(shí), bap的作用要比kt大,但2mg lbap與0 . 5mg lkt配合可獲得更佳的效果;在該細(xì)胞分裂素水平下,生長素naa用0 . 5mg l ,愈傷組織再生率最高;胚性愈傷組織先在含60g l蔗糖的分化培養(yǎng)基上高滲預(yù)分化,以及經(jīng)高滲預(yù)分化后的致密愈傷組織在瓊脂濃度為10g l的分化培養(yǎng)基上分化,能明顯促進(jìn)愈傷組織的植株再生;在分化培養(yǎng)基中添加脯氨酸導(dǎo)致愈傷組織再生率下降,但同時(shí)有減少白化苗再生的趨勢。
S . regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials , and ms as basic medium supplying different ba ^ naa and kt concentration , we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3 . 0mg / l + naa0 . 2 mg / l ) . among the three kinds of mustard explants . the cotyledons showed the highest adventitious bud regeneration capacity . and the regeneration capacity of leaf explantswas weaker . and the weakest of hypocotyls 芥菜高頻再生體系的建立以4天左右的葉用和莖用芥菜子葉、下胚軸和25天苗齡的芥菜無菌苗的葉片為外植體,以ms為基本培養(yǎng)基,通過加入不同濃度和不同組合的ba 、 naa 、 kt ,篩選到了外植體不定芽分化的最適培養(yǎng)基( ms + ba3 . 0mg / l + naa0 . 1mg / l )及生根的最適培養(yǎng)基( ms + naa0 . 2mg / l ) 。