Tnfa antagonists including mab against tnfa and tnf receptor ( tnfr ) , soluble tnfa receptor ( stnfr ) , mutant tnfa have been applied or tried to apply in the clinical with some positive therapy effects , but there were still some problems to limit the application of these agents 重組tnf及其拮抗劑(包括抗tnf單抗、抗tnf受體單抗、可溶性tnf受體、 tnf突變體)均已應用于臨床并取得了一定的治療效果。
For any other son to have stayed with his mother for four days at tr port , it would have been a condescension or a martyrdom , while i return , more contented , more peaceful - shall i say more poetic ! - than if i had taken queen mab or titania as my companion . 要是別的當兒子的陪他的母親到的黎港去住四天,他肯定會覺得枯燥,厭煩,但我陪了她四天,卻比陪伴瑪琵仙后民間傳說中的仙女,莎士比亞戲劇羅密歐與朱麗葉中有詳細描寫。
All the clones could react with swine antisera against prrsv and mouse antisera to prrsv , suggesting aa50 ~ aa55 domain on n protein might be the antigenic site recognized by mab ge3 . 4 . the gene fragment encoding the epitope recognized by mab ge3 was cloned into the expression vector pgex - 4t - 2 用prrsv陽性血清和鼠源抗prrsv抗體采用間接elisa進行鑒定,結果表明篩選到的噬菌體克隆可以與血清發生特異性反應,從而初步確定了單克隆抗體ge3所識別的抗原表位。
Studies of genes related to heart development in drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases . to clone and identify new genes that control the heart development , by a way of chemical mutagen , ems , we have established 1 , 200 balanced - lethal lines on chromosome 2 and 3 . with the screening the 330 stocks with immunochemical method using heart - specific antibody , mab . no . 3 , we detected 60 lethal lines showing heart mutant phynotype 為了克隆和鑒定控制心臟發育的新基因,本研究利用化學誘變劑甲磺酸乙酯大規模地誘變果蠅,并且建立了1200個第二和第三染色體的平衡致死系,利用心臟組織特異抗體mab . no . 3對其中330個品系進行免疫化學方法篩選,觀察到有60個致死系表現出心臟突變表型, 20個品系的心臟突變表型有待進一步證實。
24 , 48 , 72 hours later after transfection , we testified the expression of pp38 with mab h19 , and pp24 with the antiserurn of pp24 expressed in e . coli . the tests justified the expression of pp24 in prokaryotic and eukaryotic expressing systems . in order to study the correlation of pp38 and pp24 , we cloned pp38 gene and pp24 gene into pbudce4 為研究pp38和pp24之間的關系,將型mdvmd11株的pp38基因和pp24基因的完整orf克隆到真核雙表達載體pbudce4 . 1中,轉染cef后,通過ifa檢測和用抗pp24多克隆血清進行western - blotting試驗檢測到了pp38和pp24磷蛋白的共表達。
Through three rounds of screening , seventeen clones were selected and used in competitive test . the mab ge3 could specifically inhibit eight out of seventeen clones from binding to swine antisera . based on the amin acid sequences deduced from the foreign sequence inserted in the phage , it was indicated that all clones shared the core sequence - p / ekphf , that was similar to aa50 ~ aa55 domain of n protein of prrsv 從第三輪親和篩選的噬菌體中隨機挑取17個克隆進行功能鑒定,結果表明8個克隆與mabge3具有較強的特異性結合力并可以被prrsv陽性血清阻斷,測序發現7個克隆具有核心序列: p ekphf ,該序列與prrsvn蛋白aa50 aa55 ( p ekphf )具有較高的同源性。
Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5 , rhpk - 5 ) protein was recognized by mab same as native hpk - 5 . the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5 . section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system , the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5 Coli )作為宿主,經sds - page分析,篩選表達量最高的菌株作為發酵用工程菌株;用western - blot方法鑒定hpk - 5因子的免疫學活性;用搖瓶發酵的方法,研究發酵培養基的體積(溶解氧) 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響,優化hpk - 5基因工程菌的表達條件。
By using western - blot , the fusion protein could not be react with antiserum to prrsv , whereas it presented a reactivity with swine antisera against prrsv and mab ge3 by elisa the results implied that the epitope might be one conformational epitope that was mainly composed of aa50 ~ aa55 domain on n protein Western - blot分析表明表達產物與prrsv陽性血清沒有反應性,而elisa分析表明表達產物可與prrsv陽性血清和單克隆抗體發生反應。由此說明單克隆抗體ge3所識別的抗原表位可能是存在于n蛋白上以kphf為核心的構象型表位。
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii ) Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取并分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,并通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬菌體抗體庫的容量和重組率。