However , continued injection of humans with antibodies made in another species eventually leads to the production of antibodies against the foreign antibodies . this problem may now be circumvented by immunizing mice that have been transfected with large segments of the human immunoglobulin loci so the mice actually produce human antibodies V 。基因全長357hp ,屬鼠免疫球蛋白重鏈第i亞類,由種系基因v 、 dspz 8與jh4重排而來,編碼119個氨基酸殘基。
Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml , es - d3 cells being used in our experiments formed normal clones , expressed akp and kept their normal karyotype over many passages . the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers 結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因于億f )的es細胞培養液中形成典型的胚胎干細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。
Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras . the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02 , has enough polymerism and good stability , and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line , especially when involving strains that can not be discerned with coat color or biochemical markers 嘗試應用多位點dna指紋技術,檢測經過胚胎干細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明:采用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜,具有足夠的多態性和很好的穩定性。
Unicolor and lilium asiatic hybrids or cultivars in lilium asiatic hybrids were researched with their parents by karyotype , soluble protein , esterase isoenzyme and peroxidase isoenzyme . the results provided identification markers of cytology and biochemistry for hybridization at the early stage in lily breeding programs . the cluster analysis according to similarity coefficient of soluble protein and peroxidase showed that " yellow " and " omega " have the most closest relation in lilium asiatic hybrids 本研究對百合屬幾個植物的親緣關系進行了可溶性蛋白質、過氧化物酶同工酶分析,同時對亞洲百合雜交系內雜交及其與原種系間的雜交后代進行了核型、可溶性蛋白質、過氧化物酶和酯酶同工酶分析,以期為百合屬植物親緣關系分析提供生化依據,以及為雜交后代的早期鑒定提供細胞學、生化水平的檢測指標。
The optimized result of cell immobilization cultures was acquired , including the support matrices pretreatment , the class and size of support matrices , the level of dissolved 62 and inoculum . the optimal ratio of hormones was got by uniform design according to its high immobilization level , high metabolism and retaining vigorous cells with long period . a medium which fit well all the conditions was obtained , and the efficiency of producing ginkgolides by cell culture improved obviously 進行了優良種系的誘導和優選,得到了一種生長快、分散性好,并很好的符合固定化要求的細胞株系;對固定化條件進行了最優化配置- -從載體預處理、載體種類、載體量、大小和溶氧到細胞接種量系統的研究;采用均勻設計尋求一種高固定化、高代謝及長期保持細胞活力的激素配比,最終得到一種基本滿足上述要求的培養基。
2 . we use the novel copper binding protein as antigen , prepared rabbit anti rat copper binding protein polyclonal antibody . discussion recently , a number of proteins , that play significant roles in intracellular copper homeostasis , were identified in various species , ranging from bacteria to humans 討論近年來的研究發現了多種在不同生物種系維持細胞內鋼平衡的蛋白,這些蛋白分布較廣,從噬菌體到人類都存在具有這種功能的蛋白質,分為銅伴侶和銅轉運體兩種。