In immuno - blotting , these fragments reacted specifically with hepatitis c patients " sera , suggesting that e . coli - derived e2 proteins carried hcv e2 - specific , glycosylation - and - conformation - independent epitopes 在免疫印跡檢測中,上述片段與丙型肝炎病人血清有特異性反應(yīng),表明大腸桿菌系統(tǒng)表達的e2蛋白攜帶有hcve2特異的、不依賴于糖化和立體構(gòu)象的抗原決定簇。
By immunizing rabbits with aa 192 - 326 fragment , polyclonal sera were obtained that were able to recognize e1 proteins expressed in both e . coli and mammalian cells , suggesting that e . coli - derived e2 proteins carried hcv e1 - specific , glycosylation - and - conformation - independent epitopes 利用aa192 - 326免疫家兔,獲得了可識別大腸桿菌和哺乳動物細胞表達之e1蛋白的多抗血清,表明大腸桿菌系統(tǒng)表達的e1蛋白攜帶有hcve1特異的、不依賴于糖化和立體構(gòu)象的抗原決定簇。