Pbi121 was chosen as binary vector 構建了dreb1c正義植物雙元表達載體。
Finally this fusion was inserted into the binary vector pbinplus . named pbgsb 插入植物雙元表達載體pbinplus中,構建得到brazzein基因的表達載體pbgsb 。
Finally the expression cassette of the two genes were inserted into binary vector pbin19 最后得到的植物表達載體pbm含目的基因及植物選擇標記基因npt 。
From the mapping relationship between information vector matrix and relative vectors , rough approximation , attribute relative reductions and selecting optimal attribute reductions set algorithms based on binary vector matrix were proposed 摘要根據向量矩陣與向量之間的映射關系,研究了基于二元向量矩陣算法的粗糙近似、屬性約簡以及最優屬性約簡集的獲取。
They indicated that ecbp21 may have some relative with the response to stress treatments . further more , we constructed the binary vector containing ecbp21 - gfp chimeric gene and got transgenic plants by using the agrobacterium vacuum infiltration method 4 .初步開展了轉基因研究:為了進一步確定ecbp21在植物生長發育過程中的作用,構建了ecbp21植物表達二元載體,通過真空滲透法轉化了擬南芥并獲得了轉基因植株。
The modified sequence was cloned into binary vector pbi121 . the constructed expression vector was named pbi121 - bar , then transferred into agrobacterium tumefaciens lba4404 by triparental crossing . thus the project bacterium has been successfully constructed 改造后的目的基因克隆于真核表達載體pbi121 ,構建表達載體pbi121 - bar ,通過三親雜交法將真核表達載體pbi121 - bar轉入農桿菌lba4404 ,成功構建出工程菌。
The expression of tstrx from different tissue also shows different . these results show that this gene is a stress response gene or plays an important role in slat stress tolerance . aiming to identify the functions of this gene and do some further study , we have cloned the gene into the agrobacterium tumefaciens binary vector prok ii and pcambia 3013 結合實驗結果以及用200mmol . l ~ ( - 1 ) nacl處理的小鹽芥地上部分構建的zap - cdna文庫中得到的編碼硫氧還蛋白的cdna序列的相關資料推測: tstrx基因可能在保護鹽芥免受因脅迫引起的氧化損傷中起作用。