Transmission electron microscopy : the testis was cut into fragments ( 1mm x 1mm x 1mm ) , fixed in 2 . 5 % glutaraldehyde made up in 0 . 1 m phosphate buffer ( ph 7 . 2 ) , postfixed in 1 . 0 % oso4 , dehydrated in a progressive ethanol and acetone solution , embedded in epon812 , sectioned with lkb ultramicrotome , and stained with urangl acetate followed by lead citrate , then observed with h - 600 microscopy and photographed 透射電鏡樣品以2 . 5戊二醛( ph7 . 2 , 0 . 1m lpbs緩沖液配制)和1鋨酸雙固定,酒精和丙酮系列脫水, epon812包埋, lkb型超薄切片機切片,醋酸鈾和檸檬酸鉛雙重染色, h - 600透射電鏡下觀察并拍照。