Isolated microspore culture is one of the methods to obtain haploid 摘要游離小孢子培養技術是獲得大白菜單倍體植株的有效途徑之一。
Doubling efficiencies of the application dimethylsurfoxide in colchicines to treat haploids in tobacco nicotiana tabacum 對煙草單倍體植株的染色體加倍效應
Gynogenic haploids through unfertilized ovules and ovaries have been reported from 21 species in 9 families since 1976 摘要介紹被子植物未受精胚珠與子房離體培養誘導單倍體植株的研究進展。
With haploid as transgenic material can decrease mosaic and can avoid properties separation in transforming 采用單倍體植株作為遺傳轉化的材料會避免轉基因過程中的嵌合體問題和子代分離問題。
By means of pcr amplification to total dna extracted from four transgenic plants , it indicated that bet - a gene had been integrated into the haploid of the hybrid genome 最終得到四個轉化株系,經pcr檢測,全部產生特異性擴增譜帶,證明bet - a基因已整合進小黑楊單倍體植株的基因組中。
Embryological studies reveal that megaspore and such cells as egg , synergid and antipodals in mature embryo sac initiate the division of forming haploid plants through embryogenesis or callus formation 胚胎學觀察揭示大孢子與胚囊內的卵細胞、助細胞和反足細胞均有可能在培養中啟動分裂,通過胚狀體或愈傷組織形成單倍體植株。
Anther culture ( pollen culture ) the generation of haploid plants from immature pollen grains or intact excised anthers . the resultant plants are generally smaller than their diploid counterparts 花藥培養:從未成熟的花粉粒或者切下的完整的花藥中再生出單倍體植株的過程。這樣培養出來的單倍體植株通常比正常培養出的二倍體植株小。
This review includes the following subjects : induction frequency of haploids depend on genotypes , developmental stage of explants , temperature pretreatment of the explants , medium composition and the conditions of cultivation 迄今己有9個科21種植物用這一方法誘導出單倍體植株,植物的基因型、外植體的發育程度、接種前的預處理、培養基和培養條件等均能影響誘導率的高低。
Choline dehydrogenase ( cdh ) isolated from escherichia coli , can help the expression of glycine betaine efficiently . to get some salt - tolerant plants , the cdh gene , mediated by agrobacterium , was transformed to the haploid of populus simoniixp . nigra 本實驗以農桿菌( agrobacteriumtumefaciens )為介導進行遺傳轉化,將編碼cdh的耐鹽基因( bet - a )導入小黑楊( populussimonii p . nigra )單倍體植株( haploid )的基因組內,以期獲得耐鹽植株。