If the novel protein we purified has mxcxxc sequence , and what is the physical 這種蛋白質具有抗原決定簇,能免疫家兔并產生多克隆抗體。
According to the reported complete nucleotide sequence of goose parvovirus in genbank . with oligo4 . 1 . a pair of primers were designed and synthesized 與主要結構蛋白vp3存在相同的抗原決定簇成分,是制備基因工程疫苗的重要候選基因。
The protein - storing cells in swietenia macrophylla were found to be populus - type , i . e . ordinary parenchyma cells containing both vacuolar protein inclusions and starch grains 用21kda蛋白質的抗血清進行兔疫印跡分析表明, 18kda蛋白質和21kda蛋白質有相同的抗原決定簇。
The resulting derivative strains produced antifungal activity in a manner different from the parental strain , indicating the original promoter was replaced with the engineered promoters and the epitopes 衍生菌株合成抗真菌抗生素的產量與親本菌株不同,表明fr - 008基因的原始啟動子已被改造后的啟動子和抗原決定簇所代替。
Recently , people have had the ability to do some research using the technology of phage display on identification of epitope sites on surface of antigens , the diagnosis and therapy of diseases , and in any other important fields 通過噬菌體展示技術,目前人們已能借助此類文庫研究包括抗原決定簇的鑒定、疾病的診斷與治療等許多領域的諸多關鍵性問題。
Integrated plasmids containing phage lambda promoter pr - directed and epitope - tagged 2 . 7 kb pks gene were constructed for tagging the natural fr - 008 pks with specific immunodeterminant ( epitope ) . these constructs were transferred into streptomyces sp 構建了帶有噬菌體啟動子和特異性抗原決定簇(表位)及2 . 7kbpks基因的整合型質粒,用于標記天然的fr - 008pks 。
In immuno - blotting , these fragments reacted specifically with hepatitis c patients " sera , suggesting that e . coli - derived e2 proteins carried hcv e2 - specific , glycosylation - and - conformation - independent epitopes 在免疫印跡檢測中,上述片段與丙型肝炎病人血清有特異性反應,表明大腸桿菌系統表達的e2蛋白攜帶有hcve2特異的、不依賴于糖化和立體構象的抗原決定簇。
Compared relative similarity with the amino acid sequence of reported allergen protein , a peptide fragment located at the site of 183 - 188 , keeeke , was common to different allergens , so we speculated that it might act as the potential epitope Tb22kda蛋白與已知過敏原氨基酸序列比較顯示,其中183 ? 188位的序列keeeke在不同過敏原中都有存在,推測可能為潛在的抗原決定簇。
And the positive clones were used as the immunogens to immunize mice . both of the serum and phage peptides were add to measure their effects on the growth of huvec ( human umbilical vascular endothelial cell ) 檢驗這種短肽可否模擬vegf的抗原決定簇誘發機體產生能與vegf結合的抗體,研究小鼠抗血清對huvec (人臍靜脈血管內皮細胞)生長的抑制作用以及噬菌體表達的7肽和12肽對huvec生長的抑制作用。
The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest . then the recombinant plasmid was identified by endonuclease analysis , pcr ampliation and dna sequencing . the report showed that the recombinant plasmid had right open reading frame 重組質粒經酶切鑒定, pcr鑒定和測序,結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a ( + ) ,且閱讀框架正確。