Cytochemical methods : the testis was fixed in cold acetone , embedded in paraffin , sectioned at 6 u m , observed with olympus bh - 2 microscopy and dna was showed with feulgen , glycogen was showed with pas 細胞化學樣品以冷丙酮固定,常規石蠟包埋,切片厚度為6 m , feulgen反應顯示dna ; pas反應顯示糖原顆粒, olympusbh - 2型顯微鏡觀察。
The surface ladder effect produced by prototyping principle is analyzed , and the calculation method of the part surface roughness is presented . the selecting method of slice thickness and sintering layer thickness when slicing the cad model and sintering is presented 分析了由于成型原理所產生的制件表面階梯效應,提出了制件表面粗糙度的計算方法,給出了cad模型切片時,切片厚度的選取方法。
In this paper , the methods that the author used are as follows : light microscopy : the testis was fixed in bouin ' s fluid , dehydrated in an ethyl alcohol series , embedded in paraffin , sectioned at 6 u m and stained with hematoxylin and eosin , then observed with olympus microscopy and photographed 光鏡樣品以bouin ' s固定液固定,系列酒精脫水,石蠟包埋,切片厚度6 m ,蘇木精、伊紅染色, olympus顯微鏡觀察并拍照。
Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces 2 )磷酸緩沖液100400m , 30分鐘灌注完畢,取出整腦,在上述固定劑oc )內后固定12小時。切取觀察部位腦塊,然后,進行梯度酒精脫水,浸蠟,包埋,修塊,石蠟連續切片(德國leica石蠟切片機人切片厚度still , zlllll ,蛋自甘油載片撈片, 60c烤箱過夜,二甲苯脫蠟,梯度酒精置換,浸水, h六染色,梯度酒精脫水,二甲苯透明,中性樹脂封片。室溫風干后,顯微鏡觀片, olympus萬能顯微鏡照相。