May 18 , 2007 ? in the first documented us case of eczema vaccinatum ( ev ) since 1988 , reported in the may 18 issue of the morbidity and mortality weekly report , secondary transmission of vaccinia virus occurred from a father vaccinated for smallpox to his child 2007年5月18日,發病率和死亡率每周報道5月18日刊報道,美國首例牛痘性濕疹于1988年被報道,繼發的痘苗病毒傳播發生在一位接種了天花疫苗的父親傳染給了兒子。
On march 8 , real - time polymerase chain reaction orthopoxvirus generic assay and nonvariola orthopoxvirus assay were positive for orthopoxvirus dna , supporting the clinical diagnosis of ev , and the diagnosis of vaccinia was confirmed at the us centers for disease control and prevention 3月8日,實時聚合酶鏈反應痘苗病毒屬測定和非天花痘苗病毒屬測定痘苗病毒屬dna是陽性,支持牛痘性濕疹的臨床診斷,并且美國疾病控制與預防中心也證實了牛痘的診斷。
Hi our study , dendritic cells ( dcs ) were derived from the cultivation of peripheral blood monocytes in vitro successfully . then to observe whether dcs transfected with carcinoembryonic antigen ( cea ) - vaccinia recombinant virus ( rv - cea ) can induces cytotoxic t lymphocyte - mediated cea - specific immunity in vitro 體外成功地完成了cd14 +單核細胞來源的樹突狀細胞( dc )的培養,并進一步研究人癌胚抗原重組痘苗病毒( rv - cea )轉染dc后體外誘導的cea特異性細胞免疫。
In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv 本研究采用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,并獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
“ the administration of smallpox vaccine to this service member and his subsequent contact with his family are under investigation by the us military , which will determine whether screening and education practices need to be modified , ” an accompanying editorial notes . “ health - care workers treating patients with ev , generalized vaccinia infection , or progressive vaccinia infection should follow contact precautions until patients ' scabs have separated “美國軍方調查研究了對這個現役軍人和他家族后來的接觸者的牛痘苗的管理,以決定是否要篩查和修改訓練實習方案, ”跟蹤的社論報道, “健康護理工作者對待牛痘性濕疹、全身性種痘反應或進行性牛痘感染者應該隨訪給予接觸警惕直到患者痂分離。
To answer this question , a bispecific , trifunctional antibody constructs which can not only target block virus incorporated rca , but also can induce complement activation by it ' s fc fragment were designed and constructed and iv the role of this kind of bispecific antibody in virus neutralization was studied . 1 . to test our idea , human immunodeficient virus ( hiv ) and enveloped extracellular virus ( eev ) of vaccinia virus ( vv ) were selected in our study because of their complex immune evasion stratiges , their threaten to humans , and because both these two kinds of virus can escape complement attack by incorporating host rca into their envelope 以嚴重危害人類健康,且具有復雜免疫逃避機制的有包膜的hiv病毒及痘苗病毒的eev病毒為研究對象,首先對它們逃避補體攻擊的現象進行了驗證,探討了宿主膜補體調節蛋白cd55 、 cd59與hiv病毒及eev病毒免疫逃避的關系,評價了病毒結合的這兩種補體調節蛋白作為本研究提出的,通過消除病毒逃避補體攻擊的機制來恢復病毒對補體攻擊的敏感性,提高補體抗病毒效率這一抗病毒策略的靶點的可能性。
a viral disease of cattle causing a mild skin disease affecting the udder; formerly used to inoculate humans against smallpox 同義詞:cowpox,
a local infection induced in humans by inoculation with the virus causing cowpox in order to confer resistance to smallpox; normally lasts three weeks and leaves a pitted scar 同義詞:vaccina, variola vaccine, variola vaccinia, variola vaccina,