Standard test method for measuring mek resistance of ethyl silicate inorganic zinc - rich primers by solvent rub 用溶劑擦試法測定硅酸乙酯
Furthermore , when normally induced 3t3 - l1 preadipocytes were treated with pd98059 ( an inhibitor of mek - 1 ) , to block the activation of erk1 and 2 , the mitotic clonal expansion was blocked , while adipocyte differentiation was not affected 我們用有絲分裂原激活蛋白激酶激酶( mapkk / mek1 )的抑制劑pd98059與誘導(dǎo)分化激素及藥物一起對細胞誘導(dǎo)分化,克隆擴增不發(fā)生,但細胞分化未受影響。
There are two egfr signaling pathways , one is the classical ras / raf / mek / mapk pathway and the other is pi - 3 - k / pkb pathway . in this part , the phosphorylation and / or activity of some key molecules in these two signaling pathways in differently transfected h7721 cells were investigated . by means of specific antibodies combined with weste Egf刺激后不同轉(zhuǎn)染細胞中的pkb活性、 p42 / 44mapk和mek磷酸化都明顯增加,其中g(shù)ntv一s / h7721細胞pkb比活性、 p42 / 44mapk和mek磷酸化程度較mock細胞增加明顯,而gntv一as / h7721細胞中上述指標的增加程度則不及mock細胞。
These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr . it seems that the increased 1 , 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n , while the decrease of this branch may prevent these processes 用特異性抗體結(jié)合westemblot結(jié)果發(fā)現(xiàn),正義或反義gnt一vcdna的轉(zhuǎn)染并不引起pkb 、 p44 / 42mapk和mek蛋白質(zhì)表達的變化,而gntv一s / h ” 21細胞pkbt308 、 5473位點磷酸化和免疫沉淀pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化為指標的pkb的活性都較mock細胞增加, gntv一as / h7721細胞中這些指標的變化則相反。