例句與用法

1. This experiment passing to grope for the carbon source constitutes of the culture medium and using t . reesei rut c - 30 induced the expression of # - mannanase ( # - 1 , 4 - mannan mannohydrolase ec 3 . 2 . 1 . 78 ) . in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t . reesei rut c - 30 , then proceeded the variable carbon source ( dragon spruce fiber , com rush pith fiber , wheat straw fiber , wheat straw xylan , corn rush pith xylan , dragon spruce mannan ) to single factor , double factor , three factor , four factor and five factor orthogonal experiment . 1 determined the activity of p - mannanase using locost bean gum as substract by the 3 , 5 - dinitosalicylic acid method , and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber , wheat straw xylan , dragon spruce mannan
   在里氏木霉rutc - 30的基礎培養(yǎng)基( mandels營養(yǎng)液)中加入固定碳源乳糖和槐豆膠，然后將可變碳源(云杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、云杉甘露聚糖)進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養(yǎng)實驗，并以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養(yǎng)液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含云杉纖維、麥桿木聚糖和云杉甘露聚糖的誘導培養(yǎng)基為最佳培養(yǎng)基，用該培養(yǎng)基培養(yǎng)的里氏木霉( t . reesei ) rutc - 30使其轉錄的-甘露聚糖酶( - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ) mrna量能夠滿足rt - pcr的要求。
2. The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands , which exactly demonstrated at protein level that a . niger j 506 was surely a mutant of a . niger m1 . zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture , while two xylanases in initial strain . what is important , the third xylanase in a . niger j 506 have higher activity and more production levels from page and zymogram of xylanase
   尤其是在木聚糖酶譜帶檢測中發(fā)現(xiàn)，突變株發(fā)酵液中有三種類型的木聚糖酶，而出發(fā)菌株中只有兩種類型的木聚糖酶，并且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發(fā)現(xiàn)，突變株中第三種類型的木聚糖酶不僅表達量很大，活力也很高。