- 發(fā)酵罐
- 美國生產(chǎn)的一種含四環(huán)素的冰
- 生物穩(wěn)定劑
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biostat中文什么意思
例句與用法
- According to these problems , we adopt to the method of mending material , optimize to fermentation media and partly ferment condition . finally , we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified . with the plasmid pbv220 - ifnr , pbv220 - hgfa , pbv220 - hgfb , pbv220 - hpk5 that expresses serve as the model , adopting the biostat - c15l of b . braun company , utilize the method of mending material to ferment , through optimization fermentation media and optimization partly ferment condition ( ventilate quantity , stir speed , mend material speed ) , eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構(gòu)建并穩(wěn)定表達(dá)的重組質(zhì)粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達(dá)宿主菌中篩選出一種適合大規(guī)模生產(chǎn)的菌種bl21 ( de3 ) ，該工程菌株連續(xù)傳代100代表達(dá)質(zhì)粒不丟失，表達(dá)量穩(wěn)定；采用b . braun公司的biostat - c15l自控發(fā)酵罐，運(yùn)用分批補(bǔ)料技術(shù)分別進(jìn)行四種工程菌的高密度發(fā)酵，通過優(yōu)化工程菌發(fā)酵的培養(yǎng)基配方及優(yōu)化部分發(fā)酵條件(通氣量、攪拌速度、補(bǔ)料速度) ，最終建立一種適于目的基因高效表達(dá)的高密度發(fā)酵工藝模式。