A page gel , stained by the petiodic acid - schiff ' s method to reveal glycoproteins , further displayed that the bindng - protein was a glycoprotein belonging to lectin , which contained 17 . 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method . peptide mapping was comparable to the reported protein in protein bank . the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase , aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b , glutamyl - trna reductase , histidyl - trna synthetase 連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 “ . skda ,由二個相同的亞基組成,亞基分子量為” . ikda ,等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白,其中糖含量為17 . 44 % ,蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素
百科解釋
In enzymology, a L-aspartate oxidase () is an enzyme that catalyzes the chemical reaction